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2023 2

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2020 1

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他克莫司 1

代谢组学 1

宏基因组 1

比较基因组 1

深古菌 1

移植后糖尿病 1

红基因组学 1

超嗜热适应性 1

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Culturomics and metagenomics: In understanding of environmental resistome

Monika Nowrotek, Łukasz Jałowiecki, Monika Harnisz, Grażyna Anna Płaza

《环境科学与工程前沿（英文）》 2019年第13卷第3期 doi: 10.1007/s11783-019-1121-8

摘要：

State of the art of culturomics and metagenomics to study resistome was presented.

The combination of culturomics and metagenomics approaches was proposed.

The research directions of antibiotic resistance study has been suggested.

关键词： Culturomics Metagenomics Antibiotic resistance Resistome

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METAGENOMICS COMBINED WITH HIGH-THROUGHPUT SEQUENCING REVEALS THE METHANOGENIC POTENTIAL OF FRESH CORN

《农业科学与工程前沿（英文）》 2023年第10卷第3期页码 403-423 doi: 10.15302/J-FASE-2022471

摘要：

● Methane production from fresh straw was 7.50% higher than dry straw.

关键词： fresh corn straw high solid anaerobic digestion metagenomics microbial communities thermophilic

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Distribution of antibiotic resistance genes and their association with bacteria and viruses in decentralized sewage treatment facilities

《环境科学与工程前沿（英文）》 2022年第16卷第3期 doi: 10.1007/s11783-021-1469-4

摘要：

• Distribution of ARGs in decentralized sewage facilities were investigated.

关键词： Decentralized sewage treatment facilities Antibiotic resistance genes Virus Metagenomics Network analysis

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深古菌门的核心代谢功能和热环境起源 Article

冯晓远, 王寅炤, Rahul Zubi, 王风平

《工程（英文）》 2019年第5卷第3期页码 498-504 doi: 10.1016/j.eng.2019.01.011

摘要：

深古菌门具有复杂的亚群分类，是地球上丰度最高的微生物之一。然而，深古菌的代谢特征和演化历史仍然有待进一步研究。本研究对来自10个不同亚群的15个新获得和36个已经发表的深古菌基因组进行了比较基因组分析，揭示了深古菌门的核心代谢特征，即蛋白质、脂质、芳香族化合物降解，糖酵解途径和Wood–Ljungdahl (WL) 途径。上述核心代谢特征表明深古菌使用乙酰辅酶A作为重要的代谢中间物。此外，部分深古菌亚群还具有不完整的柠檬酸循环、产乙酸途径和硫化物相关的代谢潜能，表明不同亚群具有多样的代谢能力和生态功能。亚群Bathy-21和Bathy-22位于深古菌系统发育树根部，是目前已知最古老的两个深古菌亚群。它们广泛分布于热液（泉）环境中，并编码超嗜热适应性特征的标记基因逆促旋酶（reverse gyrase, rgy）。综上，本研究对深古菌门的核心代谢能力进行了系统性研究和总结，并揭示了深古菌热环境起源的演化历史。

关键词：深古菌宏基因组比较基因组超嗜热适应性

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Taxonomic and functional variations in the microbial community during the upgrade process of a full-scale landfill leachate treatment plant – from conventional to partial nitrification-denitrification

Binbin Sheng, Depeng Wang, Xianrong Liu, Guangxing Yang, Wu Zeng, Yiqing Yang, Fangang Meng

《环境科学与工程前沿（英文）》 2020年第14卷第6期 doi: 10.1007/s11783-020-1272-7

摘要： Abstract • Upgrade process was investigated in a full-scale landfill leachate treatment plant. • The optimization of DO can technically achieve the shift from CND to PND process. • Nitrosomonas was mainly responsible for ammonium oxidation in PND system. • An obviously enrichment of Thauera was found in the PND process. • Enhanced metabolic potentials on organics was found during the process update. Because of the low access to biodegradable organic substances used for denitrification, the partial nitrification-denitrification process has been considered as a low-cost, sustainable alternative for landfill leachate treatment. In this study, the process upgrade from conventional to partial nitrification-denitrification was comprehensively investigated in a full-scale landfill leachate treatment plant (LLTP). The partial nitrification-denitrification system was successfully achieved through the optimizing dissolved oxygen and the external carbon source, with effluent nitrogen concentrations lower than 150 mg/L. Moreover, the upgrading process facilitated the enrichment of Nitrosomonas (abundance increased from 0.4% to 3.3%), which was also evidenced by increased abundance of amoA/B/C genes carried by Nitrosomonas. Although Nitrospira (accounting for 0.1%–0.6%) was found to stably exist in the reactor tank, considerable nitrite accumulation occurred in the reactor (reaching 98.8 mg/L), indicating high-efficiency of the partial nitrification process. Moreover, the abundance of Thauera, the dominant denitrifying bacteria responsible for nitrite reduction, gradually increased from 0.60% to 5.52% during the upgrade process. This process caused great changes in the microbial community, inducing continuous succession of heterotrophic bacteria accompanied by enhanced metabolic potentials toward organic substances. The results obtained in this study advanced our understanding of the operation of a partial nitrification-denitrification system and provided a technical case for the upgrade of currently existing full-scale LLTPs.

关键词： Landfill leachate Process upgrade Partial nitrification-denitrification Bacterial community Metagenomics

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肝脏移植术后糖尿病患者肠道微生物组的变化 Article

凌琪, 韩玉秋, 马越, 王晓森, 朱铮, 王靖宇, 曹佳莹, 林笑含, 王军, 王保红

《工程（英文）》 2023年第31卷第12期页码 98-111 doi: 10.1016/j.eng.2023.09.006

摘要：

移植后糖尿病（PTDM）会增加肝移植（LT）受者移植失败和死亡的风险。实验研究表明免疫抑制性他克莫司（TAC）介导的肠道生态失调可能导致血糖紊乱，但仍尚未见相关临床研究报道。因此，本研究通过高通量鸟枪法宏基因组测序和代谢组学分析相结合的方法，对PTDM和非PTDM的肝脏移植受者队列中的肠道微生物组（IM）进行了表征，并破译了IM、TAC剂量和PTDM之间的潜在关联。通过与非 PTDM 和经典II型糖尿病（T2DM）进行对比分析，确定了PTDM患者的肠道微生物组代表性特征为：变形菌门富集和拟杆菌门减少。有趣的是，与改变的微生物代谢产物相一致，这些微生物在PTDM患者中的丰都变化与TAC的剂量显著关联。具体来说，与低TAC 谷浓度（< 5 ng∙mL^–1）的移植受体相比较，高 TAC 谷浓度（> 5 ng∙mL^–1）移植受者中的有益微生物水平被显著降低；同时，通过检测粪便代谢物发现其中参与α-亚麻酸和花生四烯酸-这些降低糖尿病发生的有益代谢物的显著降低。更进一步，这些微生物标签分子的变化与移植受者的血糖紊乱程度显著有关。总之，PTDM患者的粪便微生物组和代谢组发生了显著变化，且与 TAC 剂量相关。这项研究是首个探索PTDM患者的细菌分类学组成变化和细菌基因功能变化，以更好地理解IM对PTDM的影响。

关键词：移植后糖尿病他克莫司红基因组学代谢组学